Antibody Validations
Antibody Validations
Featured Article
Opinion Article
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The challenges with the validation of research antibodies
[version 1; peer review: 4 approved]
Peer Reviewers
Alison H. Banham;
Fridtjof Lund-Johansen;
Michael G. Weller;
Simon Glerup
Antibody Validation Article
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Revised
Validation of a commercial antibody to detect endogenous human nicastrin by immunoblot
[version 2; peer review: 2 approved, 1 approved with reservations]
Peer Reviewers
Satoru Funamoto;
Lucia Chavez-Gutierrez;
Jongkyun Kang
- Funders
- National Center for Advancing Translational Sciences
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
Antibody Validation Article
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Revised
Comparative study of commercially available and homemade anti-VAMP7 antibodies using CRISPR/Cas9-depleted HeLa cells and VAMP7 knockout mice
[version 2; peer review: 2 approved]
Peer Reviewers
Alison H. Banham;
Marc G. Coppolino and Olivia Grafinger
- Funders
- Agence Nationale de la Recherche
- Association Robert Debré
- École des Neurosciences de Paris
- Fondation pour la Recherche Médicale
- AFM-Téléthon
- Fondation Bettencourt Schueller
Editorial
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NOT PEER REVIEWED
The 3rd Antibody Validation meeting: Bath UK 20-21st September 2018
[version 1; peer review: not peer reviewed]
Antibody Validation Article
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Revised
Validation of a yeast malate dehydrogenase 2 (Mdh2) antibody tested for use in western blots
[version 2; peer review: 2 approved]
Peer Reviewers
Andreas Hartig;
Ewald H Hettema
- Funders
- European Research Council
- Deutsche Forschungsgemeinschaft
Antibody Validation Article
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Revised
Validation of commercial ERK antibodies against the ERK orthologue of the scleractinian coral Stylophora pistillata
[version 2; peer review: 3 approved]
Peer Reviewers
Andrea Pitzschke;
Immacolata Castellano;
María L. Parages
- Funders
- Université Pierre et Marie Curie
- Centre Scientifique de Monaco
Opinion Article
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The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data
[version 1; peer review: 2 approved, 2 approved with reservations]
Peer Reviewers
Steven Elliott;
Alison H. Banham;
C. Glenn Begley;
Andrew D. Chalmers
Antibody Validation Article
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Revised
Referencing cross-reactivity of detection antibodies for protein array experiments
[version 2; peer review: 2 approved, 1 approved with reservations]
Peer Reviewers
Brigitte Hantusch;
Carsten Grötzinger;
Konrad Büssow
- Funders
- Enterprise Ireland
- Science Foundation Ireland
- Irish Cancer Society
Antibody Validation Article
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Revised
Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence
[version 2; peer review: 2 approved]
Peer Reviewers
Maria Laura Allende;
Dagmar Meyer Zu Heringdorf
- Funders
- Fay Fuller Foundation
- Royal Adelaide Hospital Dawes Scholarship
- National Health and Medical Research Council
- University of South Australia
- Australian Federal Government
Antibody Validation Article
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Evaluation of recombinant monoclonal antibody SVmab1 binding to NaV1.7 target sequences and block of human NaV1.7 currents
[version 1; peer review: 3 approved]
Peer Reviewers
David Hackos;
Alan D. Wickenden and Wilson Edwards;
Theodore Cummins
Antibody Validation Article
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Revised
Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle
[version 2; peer review: 3 approved]
Peer Reviewers
Naohiro Hashimoto;
Omar Skalli;
Shoichiro Ono
Antibody Validation Article
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Revised
Optimized purification strategies for the elimination of non-specific products in the isolation of GAD65-specific monoclonal autoantibodies
[version 2; peer review: 2 approved, 1 approved with reservations]
Peer Reviewers
David Soll;
Xiao He;
Christiane Hampe
Antibody Validation Article
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Revised
Antibody performance in ChIP-sequencing assays: From quality scores of public data sets to quantitative certification
[version 2; peer review: 2 approved]
Peer Reviewers
Antonio Hurtado;
Jason S. Carroll and Adam W. Nelson
Opinion Article
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siRNA knockdown validation 101: Incorporating negative controls in antibody research
[version 1; peer review: 3 approved]
Peer Reviewers
Md. Golam Sharoar;
Angus C. Nairn;
Shi-Jiang Lu
Antibody Validation Article
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Validation of a commercially available anti-REDD1 antibody using RNA interference and REDD1-/- mouse embryonic fibroblasts
[version 1; peer review: 2 approved]
Peer Reviewers
Peter Cavnar;
Mei Yee Leung
Opinion Article
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Peer Reviewers
C. Fernando Valenzuela;
Andrew D. Chalmers;
Angus C. Nairn
Opinion Article
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Revised
Quality of histone modification antibodies undermines chromatin biology research
[version 2; peer review: 3 approved]
Peer Reviewers
Andrew Bradbury;
Scott B. Rothbart;
Shohei Koide
Antibody Validation Article
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Reactivity of vertebrate-directed phospho-eEF2 antibody against the Caenorhabditis elegans orthologue phospho-EEF-2
[version 1; peer review: 2 approved]
Peer Reviewers
Richard Silva;
Kevin N. Dalby
Opinion Article
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How to avoid pitfalls in antibody use
[version 1; peer review: 2 approved]
Peer Reviewers
Simon Glerup;
Anita Bandrowski
Antibody Validation Article
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Revised
Immunoblotting validation of research antibodies generated against HS1-associated protein X-1 in the human neutrophil model cell line PLB-985.
[version 2; peer review: 3 approved]
Peer Reviewers
Andrew D. Chalmers;
Mautusi Mitra;
Lawrence L. LeClaire III